Table 1.
Gene Analysis of the Effects of L + E + P on PC3 Cells.
| Gene Name | Product | Fold Change | Function |
| CDKN2B | Cyclin-dependent kinase inhibitor 2B; p15 | →4.9 | Cell cycle control |
| CLDN1 | Claudin 1 | →3.6 | Adhesion |
| CDKN2A | Cyclin-dependent kinase inhibitor 2A; p16 | →2.2 | Cell cycle control |
| CDKN1A | Cyclin-dependent kinase inhibitor 1A; p21 | →2 | Cell cycle control |
| PTEN | Pten | →1.6 | Tumor suppressor |
| CCNE2 | Cyclin E2 | ↓18 | Cell cycle control |
| ANLN | Anillin | ↓16 | Migration |
| HMMR | HA-mediated motility receptor | ↓8 | Migration |
| DTL | Denticleless homolog | ↓7 | Cell cycle control |
| CCNB1 | Cyclin B1 | ↓5.1 | Cell cycle control |
| CCNB2 | Cyclin B2 | ↓4.5 | Cell cycle control |
| ROCK2 | Rock2 | ↓4 | Migration |
| ZEB1 | Zinc finger E-box binding homeobox 1 | ↓3 | EMT |
| TWIST | Twist | ↓2.6 | EMT |
| CDC25B | Cell division cycle 25 homolog B | ↓2.5 | Cell cycle control |
| EZH2 | Enhancer of zeste homolog 2 | ↓2.3 | Oncogenic |
| NEXN | Nexilin | ↓2.3 | Migration |
| BCL2 | Bcl2 | ↓2 | Antiapoptotic |
| COL1A1 | Collagen 1 | ↓2 | Migration |
| CDK6 | Cyclin-dependent kinase 6 | ↓1.8 | Cell cycle control |
| FSCN1 | Fascin 1 | ↓1.7 | Migration |
L + E + P changes the expression profile of genes involved in the cell growth, cytoskeleton, and cell adhesion machinery. RNA was extracted from PC3 cells that had been treated with L + E + P at 8 µg/ml for 12 hours, and Affymetrix array analysis was performed as described in Materials and Methods. Relative mRNA levels are presented as fold change compared with untreated controls.