Figure 4.

Roles of Rv3132c, Rv3133c, and Rv3134c in hypoxic acr expression. (A) Quantitation of acr induction by lux assay. Cultures of rolling, log-phase BCG Montreal (black bars), BCGΔ3132 (stippled bars) and BCG:12Δ3134 (gray bars) were transformed with the acr-lux reporter construct and were either allowed to settle in 15- ml Falcon tubes overnight (static) or were placed for two hours in sealed tubes flushed with 0.2% O₂ in N₂ as described in Fig. 1. Plotted is the fold induction relative to the time 0 rolling culture. Shown are representative data from one of two experiments. (B) Reverse transcription-PCR analysis of RNA expression from Rv3132c, Rv3133c, and Rv3134c in wild-type and mutant H37Rv. RNA from each strain (indicated above each lane) was isolated. Expected size of each product: Rv3132, 278 bp; Rv3133, 299 bp; Rv3134, 206 bp; 16S RNA, 309 bp.