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. 2012 Oct 12;3:368. doi: 10.3389/fmicb.2012.00368

FIGURE 2.

FIGURE 2

Complementation assay for LytB activity. Growth curve of lytB- E. coli complemented with Rv1110 (circles) or Rv3382c (squares). These bacteria were grown to saturation, then diluted 1/100 into fresh media containing 0.5 mM IPTG. After 60 min, cultures were diluted 1/50 in media containing selective antibiotic and 0.02% glucose (to suppress endogenous LytB expression). After 220 min, cultures were diluted 1/100 in media containing selective antibiotic, but 0.2% arabinose instead of glucose, which activated expression of the endogenous LytB, to demonstrate that the Rv3382c gene itself did not have any deleterious effects (i.e., as both recombinant strains then exhibited equivalent growth).