Fig. 10.

(A) Disruption of LEDGF Sumoylation motif, K364 (K to R) promoted hLECs growth and survival at normal physiological temperature, but failed to do so during heat stress. Cells were transfected with pEGFP-vector, pEGFP-LEDGF or pEGFP-K364R plasmid. After 48 h, MTS assay was performed (A, gray bars). The data represent the mean ± SD from three independent experiments. In a parallel experiment, cells transfected with the above constructs were subjected to heat stress (43°C for 1h), and viability was monitored with MTS assay (A, black bars) following a recovery period. Transfection efficiency was normalized with values of EGFP. The data represent the means ± SD from three independent experiments (* p<0.05; **p<0.001).

(B) Influence of Sumo1 on hLECs growth and survival at normal physiological temperature or under heat stress. Cells were transfected with pEGFP-vector, pEGFP-Sumo1 or pFlag-Senp-1 as indicated. Cells were submitted for MTS assay after 48h of transfection with (black bars) or without (gray bars) heat stress. The data represent the mean ± SD from three independent experiments (* p<0.05).