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Sumo1 motif (K364) within the IBD domain of COOH-LEDGF was evolutionarily well conserved. Bioinformatics analysis and SUMOplot, a web-based program, were used to evaluate Sumo1 conjugation motifs present in LEDGF protein. Sequence alignment of human, bovine, mouse, chicken and xenopus LEDGF protein was done to identify evolutionarily conserved Sumoylation sites (ClustalW). Important lysine residues are indicated in red bold (Score; <90).
