Skip to main content
. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: FEBS J. 2012 Jul 16;279(17):3048–3070. doi: 10.1111/j.1742-4658.2012.08686.x

Fig. 7.

Fig. 7

(A) Senp-1 increased the DNA-binding activity of LEDGF. Nuclear extracts isolated from cells transfected with pFlag-Senp-1, pEGFP-Sumo1 or plasmid vector were incubated with 32p-labeled wild type probe (left panel) or its mutant (right panel) and then processed for gel-shift mobility assay. The effects of Sumo1 (lane 2) and Senp-1 (lane 1) on LEDGF DNA binding compared to control (lane 1) are shown. Cm1 indicates DNA and LEDGF complex. (B) Right graph indicates binding intensity (in pixels).