Table 2. Apparent thermal denaturation temperatures (Tm app) of the ternary complexes formed by the p27-KID mutants on binding the Cdk2/cyclin A complex.
Complex | Tm app (°C) |
p27-KIDwt/Cdk2/cyclin A | 82.3±0.5 |
p27-KID+H/Cdk2/cyclin A | 80.3±0.2 |
p27-KID−H/Cdk2/cyclin A | 78.5±0.6 |
p27-KIDloop/Cdk2/cyclin A | 73.0±0.3 |