Table 2. Genetic changes during adaptation, relative to wild-type T7.

Nucleotide	Gene	Change	Gene function	T7A	T7E	G3	G8	G12
1257–2736	0.3–0.7 fusion,	Deletion	Variousa	+	+	+	+	+
	0.4–0.6 deletion
15094	5	G->T A248S	DNA Polymerase	+	+	0.745	0.928	0.925
24088	10B	G->A E375K	Minor coat protein	+	+	+	+	+
30861	16	A->C Q89H	Internal core protein	+	+	+	+	+
30945	16	T->G N117K	Internal core protein	+	+	+	+	+
32860	16	G->A G756S	Internal core protein	+	+	+	+	+
34975	17	A->G T118A	Tail fiber	+	+	+	+	+
36492	17.5	T->G I50S	Suspected holin	+	+	+	+	+
189	Terminal repeat	C->T			−	−	0.051	0.028
3456	1	C->T R96C	RNA polymerase		−	−	−	0.029
4886	1	G->A G572G	RNA polymerase		−	−	−	0.037
7435	1.3	C->T P321S	DNA ligase		−	−	0.107	0.054
8108	1.6	C->T A68V	Non-essential, unknown		−	−	0.545	0.484
11152	3.5	C->T D149D	Lysozyme, regulation		−	−	0.123	0.085
12565	4A	G->A G334D	Helicase		−	−	−	0.026
19032	6.7	G->A E57K	Adsorption, virion head protein	+	−	0.85	0.955
19923	7.7	G->A V26M	Non-essential, unknown		−	−	−	0.025
20240	7.7	A->G (ochre to amber)	Non-essential, unknown		−	0.288	0.047	0.058
21027	8	C->T S263F	Head-tail connector		−	−	−	0.036
24537	11	T->C Y104H	Tail protein		−	0.047	−	−
27135	12	C->T T765I	Tail protein		−	−	0.034	0.039
27574	13	A->G M90V	Internal head protein		−	0.204	0.045	0.081
29770	15	G->A K482K	Internal core protein		−	0.257	0.058	0.05
30719	16	C->A P42H	Internal core protein		+	0.029	0.693	0.705
35083a	17	C->T L154L	Tail fiber		+	0.228	0.092	0.138
35385a	17	G->A Q254Q	Tail fiber		+	−	0.036	0.043
36093	17	C->T L490L	Tail fiber		−	−	0.039	−
39566a	After 19.5	C->A			+	0.306	0.076	0.056

^aindicates noncoding change.

+indicates mutation present at greater than 97.5%.

−indicates mutation present at lower than 2.5%.