Figure 5.

Germination of CAX mutant seedlings in response to abscisic acid (ABA). The germination of seeds from Col-0 (wild type) and cax knockout mutant plants was quantified after 24 h on 0.1 µM ABA. Approximately 100 seeds of each line were sterilized and sown on 1% agar plates containing ABA. After a 2-d incubation period at 4°C plates were moved to an environmentally-controlled growth chamber at 22°C. Radicle emergence from the testa was taken as the indicator of germination. Bars indicate the mean±SE percentage of germination. ** (P<0.01) denotes significant difference between CAX mutant lines and Col-0 control as determined by one-way ANOVA.