Table 1. Clinico-pathological characteristics and CFC-recovery following immunomagnetic cell separation.
Patient | Age | PSA | Prior Treatment | Clinical stage | Gleason/Bone scan mets | No. of cores involved | % core involvement (range) | CD49f CFC-recovery % | CD44 CFC-recovery % | CD133 CFC-recovery % |
1 | 81 | 50 | nil | T3 | 7/N | 10/12 | 30–100 | 92 (36/39) | 9 (6/69) | 2 (1/42) |
2 | 72 | 478 | nil | T3 | 8/Y | 6/6 | 80–100 | 89 (25/28) | 30 (10/36) | 0 (0/20) |
3 | 73 | 55 | AB | T3 | 8/N | 6/12 | 50–100 | 96 (25/26) | 6 (2/35) | 3 (1/40) |
4 | 76 | 66 | nil | T3 | 9/N | 11/12 | 30–90 | 86 (61/71) | 28 (10/35) | 0 (0/46) |
5 | 68 | 92 | nil | T3 | 9/N | 10/12 | 20–90 | 90 (52/58) | * | 6 (2/36) |
Mean ±SD | 74±5 | 148±185 | 90.6±3.7 | 18.3±12.5 | 2.2±2.5 |
All patients had Gleason score 7 or above, clinical stage T3, and one patient had metastatic disease at presentation. CFC-recovery (defined as the proportion of total CFCs within the positive fraction) was measured for each marker, with CD49f demonstrating the highest recovery.
AB = androgen ablation, SD = standard deviation.
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