Table 1. Molar ratios of OVA to fusion protein and coupling yield for the conjugation.
Study A | Study B | ||
PPM | PPM | CP | |
Amount fusion protein reduced | 48 nmol | 67 nmol | 41 nmol |
Amount fusion protein in conjugation | 48 nmol | 54 nmol | 41 nmol |
Amount OVA activated | 237 nmol | 237 nmol | 212 nmol |
Amount LC-SPDP linker | 6 µmol | 6 µmol | 5.4 µmol |
Linker∶OVA ratio | 25∶1 | 25∶1 | 25∶1 |
Amount OVA* in conjugation | 192 nmol | 214 nmol | 164 nmol |
OVA*∶fusion protein molar ratio | 4∶1 | 4∶1 | 4∶1 |
Yields | |||
OVA-fusion protein | 70% | 22% | 17% |
OVA-fusion protein | Not analysed | 76% | 46% |
OVA∶fusion protein molar ratio | - | 1.1∶1 | 1.5∶1 |