Table 1. Molar ratios of OVA to fusion protein and coupling yield for the conjugation.
| Study A | Study B | ||
| PPM | PPM | CP | |
| Amount fusion protein reduced | 48 nmol | 67 nmol | 41 nmol |
| Amount fusion protein in conjugation | 48 nmol | 54 nmol | 41 nmol |
| Amount OVA activated | 237 nmol | 237 nmol | 212 nmol |
| Amount LC-SPDP linker | 6 µmol | 6 µmol | 5.4 µmol |
| Linker∶OVA ratio | 25∶1 | 25∶1 | 25∶1 |
| Amount OVA* in conjugation | 192 nmol | 214 nmol | 164 nmol |
| OVA*∶fusion protein molar ratio | 4∶1 | 4∶1 | 4∶1 |
| Yields | |||
| OVA-fusion protein | 70% | 22% | 17% |
| OVA-fusion protein | Not analysed | 76% | 46% |
| OVA∶fusion protein molar ratio | - | 1.1∶1 | 1.5∶1 |