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. 2012 Oct 12;7(10):e47312. doi: 10.1371/journal.pone.0047312

Figure 2. Riluzole decreases AKT phosphorylation and activity. A.

Figure 2

Riluzole decreases AKT phosphorylation. Serum-starved melanoma cells were incubated in the absence (−) or presence (+) of 25 µM of riluzole for 4 and 8 hours. Immunoblots were performed using antibodies against AKT phosphorylated at serine 473 (pAKT (S473)) or at threonine 308 (pAKT (T308)). Anti-total AKT and GAPDH antibodies were used for normalization. B. AKT-mediated GSK3 phosphorylation is decreased after riluzole treatment. Serum-starved melanoma cells were incubated in the absence (−) or presence (+) of 25 µM of riluzole for 4, 8 and 16 hours. Immunoblots were performed using antibodies against GSK3β phosphorylated at serine 9, an AKT site; total GSK3β; GAPDH.