Table 2. Primer classification, polymorphism screening in parents, and amplification in DH population.

Classificationa	Primers prefixb	Typec	No.d	Polymorphisme	Amplificationf	Markersg
	BN	SSR	24	2	1	2
	BnEMS, BnGMS, BoGMS, BrGMS	SSR	678	112	66	74
	BRAS, CB	SSR	310	40	21	24
	CNU	SSR	187	58	27	32
	EJU,ENA	SSR	25	5	3	3
	FITO	SSR	231	26	15	16
Public	IGF	SSR	95	11	4	4
	MR	SSR	23	5	2	3
	Na,Ni,Ol,Ra	SSR	398	59	30	34
	Niab	SSR	144	48	22	24
	sN, sR, sS	SSR	129	34	15	18
	Subtotal		2244	400	206	234
	BrSF	SSR	893	213	81	104
	BrBAC, P	SSR	378	91	53	63
	BoSF, SF	SSR	2422	289	169	181
Genome	Snap	SNP	2086	101	40	40
	Pr	SNP	71	9	5	5
	Ns	SNP	94	15	3	3
	Subtotal		5944	718	351	396
	GSSR	SSR	180	41	34	35
	GIFLP	IFLP	107	11	8	9
Gene	GSNP	SNP	42	10	6	6
	Gindel,GTFzip	INDEL	114	11	8	9
	Subtotal		443	73	56	59
	Total		8631	1191	613	689

^aPrimer classifications: Public indicates publicly available primers, Genome indicates newly developed genome-sourced primers, Gene indicates newly developed primers related to oil-content genes.

^bPrefix for each primer when it was designed.

^cPrimer type based on its design.

^dTotal number of primers.

^eTotal number of polymorphic primers screened in parents zy036 and 51070.

^fTotal number of polymorphisms amplified by primers in DH population.

^gTotal number of molecular markers used to construct genetic linkage map in DH population.