Table 2. Primer classification, polymorphism screening in parents, and amplification in DH population.
Classificationa | Primers prefixb | Typec | No.d | Polymorphisme | Amplificationf | Markersg |
BN | SSR | 24 | 2 | 1 | 2 | |
BnEMS, BnGMS, BoGMS, BrGMS | SSR | 678 | 112 | 66 | 74 | |
BRAS, CB | SSR | 310 | 40 | 21 | 24 | |
CNU | SSR | 187 | 58 | 27 | 32 | |
EJU,ENA | SSR | 25 | 5 | 3 | 3 | |
FITO | SSR | 231 | 26 | 15 | 16 | |
Public | IGF | SSR | 95 | 11 | 4 | 4 |
MR | SSR | 23 | 5 | 2 | 3 | |
Na,Ni,Ol,Ra | SSR | 398 | 59 | 30 | 34 | |
Niab | SSR | 144 | 48 | 22 | 24 | |
sN, sR, sS | SSR | 129 | 34 | 15 | 18 | |
Subtotal | 2244 | 400 | 206 | 234 | ||
BrSF | SSR | 893 | 213 | 81 | 104 | |
BrBAC, P | SSR | 378 | 91 | 53 | 63 | |
BoSF, SF | SSR | 2422 | 289 | 169 | 181 | |
Genome | Snap | SNP | 2086 | 101 | 40 | 40 |
Pr | SNP | 71 | 9 | 5 | 5 | |
Ns | SNP | 94 | 15 | 3 | 3 | |
Subtotal | 5944 | 718 | 351 | 396 | ||
GSSR | SSR | 180 | 41 | 34 | 35 | |
GIFLP | IFLP | 107 | 11 | 8 | 9 | |
Gene | GSNP | SNP | 42 | 10 | 6 | 6 |
Gindel,GTFzip | INDEL | 114 | 11 | 8 | 9 | |
Subtotal | 443 | 73 | 56 | 59 | ||
Total | 8631 | 1191 | 613 | 689 |
Primer classifications: Public indicates publicly available primers, Genome indicates newly developed genome-sourced primers, Gene indicates newly developed primers related to oil-content genes.
Prefix for each primer when it was designed.
Primer type based on its design.
Total number of primers.
Total number of polymorphic primers screened in parents zy036 and 51070.
Total number of polymorphisms amplified by primers in DH population.
Total number of molecular markers used to construct genetic linkage map in DH population.