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. 2001 Jun 19;98(13):7552–7557. doi: 10.1073/pnas.121188598

Table 1.

Putative function and expression analysis of 12 sequenced GAS genes

SPy no.d Putative functione LPXTG(L)f Expression analysisa
Versus wild type, exponential phaseb
Versus wild type, stationary phasec
Wild-type stationaryg covR mutant exponentialh mga mutant exponentiali covR mutant stationaryj mga mutant stationaryk
2211 Transmembrane protein Yes 1.78 1.91 2.32 0.68 0.37
1986 Phosphotransferase enzyme II, ABC component Yes 70.71 1.24 2.81 0.09 0.89
1972 Amylopullulanase Yes 31.91 1.42 0.63 0.07 1.10
1858 XAA-PRO dipeptidyl peptidase No 1.74 1.31 1.33 0.46 0.48
1857 Listeriolysin regulatory protein homolog No 1.78 1.91 2.32 0.68 0.37
1361 Internalin homolog No 2.07 1.03 0.67 1.26 0.59
1239 Aminopeptidase N Yes 0.99 1.59 1.11 0.98 1.69
0872 2′,3′-cyclic-nucleotide 2′-phosphodiesterase Yes 4.34 1.70 2.06 0.74 0.84
0843 Cell surface proteinl Yes 2.97 2.14 2.67 0.52 1.23
0747 Extracellular nuclease Yes 16.35 3.54 2.44 0.93 1.97
0501 Multidrug exporter No 1.50 1.16 3.00 0.48 1.91
0277 Amino acid ABC transporter permease component Yes 0.58 0.99 0.89 0.97 0.63
a

As assayed by RT–PCR (TaqMan). Values presented are the normalized proportional increase (>1.0) or decrease (<1.0) in transcript abundance. 

b

Expression compared to that in wild-type M1 GAS (MGAS 5005) isolated in the exponential phase of growth. 

c

Expression compared to that in wild-type M1 GAS (MGAS 5005) isolated in the stationary phase of growth. 

d

SPy number refers to the corresponding numerical identifier of each ORF as listed in GenBank. The genomic position of each ORF is as follows: spy2211, 1,845,056-1,847,632; spy1986, 1,654,795-1,656,981; spy1972, 1,639,165-1,642,662; spy1858, 1,541,907-1,544,189; spy1857, 1,541,166-1,541,885; spy1361, 1,127,063-1,129,441; spy1239, 1,020,347-1,022,884; spy0872, 718,489-720,501; spy0843, 694,643-697,669; spy0747, 607,542-610,274; spy0501, 402,159-403,352; spy0277, 241,807-243,375. 

e

As determined by WIT2 or blast analysis. 

f

Amino acid motif used to anchor proteins to the cell wall in gram-positive organisms. 

g

Wild-type M1 GAS (MGAS 5005) isolated in the stationary phase of growth. 

h

CovR-negative isogenic mutant of M1 GAS (MGAS 5005) isolated in the exponential phase of growth. 

i

Mga-negative isogenic mutant of M1 GAS (MGAS 5005) isolated in the exponential phase of growth. 

j

CovR-negative isogenic mutant of M1 GAS (MGAS 5005) isolated in the stationary phase of growth. 

k

Mga-negative isogenic mutant of M1 GAS (MGAS 5005) isolated in the stationary phase of growth. 

l

blast results indicate homology to a cell surface antigen of Bacteroides forsythus (GenBank no. T31094), a choline-binding protein of Streptococcus pneumoniae (GenBank no. CAB04758), and internalin B of Listeria ivanovii (GenBank no. CAC20606).