Figure 2.

Calibration of microfluidic chip. (a) Changes in resistance across the gap in empty chamber when the central channel was repeatedly perfused with sucrose solution with and without 2-APB (200 μM), showing that a repeatable gap resistance can be attained with multiple perfusions and that the addition of drug does not alter the gap resistance. (b) A stable gap resistance is maintained during solution exchange in central stream. (c) Changes in resistance across the gap when the central channel was perfused with sucrose solution in the presence of confluent cells. The ground resistance in (a) and (c) is the chamber resistance without sucrose gap.