Fig. 2.

In vitro activity of mutant hRFT3. a Uptake of [³ H]riboflavin by HEK293 cells transfected with empty vector, SLC52A2^wt (WT), SLC52A2^368T>C (L123P), and SLC52A2^1016T>C (L339P). The cells were incubated in buffer (pH 7.4) containing 10 nM [³ H]riboflavin for 1 min at 37 °C. Each bar represents the mean ± SEM, n = 3. Data were analyzed by Dunnett’s two-tailed test after one-way ANOVA. ** Different from vector-transfected cells, P < 0.01. ## Different from SLC52A2^wt-transfected cells, P < 0.01. b RNA expression of SLC52A2 in HEK293 cells transfected with empty vector, SLC52A2^wt (WT), SLC52A2^368T>C (L123P), and SLC52A2^1016T>C (L339P). RT-PCR analysis was carried out using specific primer sets. Products of 360 bp that corresponded to the SLC52A2 gene were separated by 1.5 % agarose gel electrophoresis and visualized by ethidium bromide staining