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. 2011 Mar;25(3):830–839. doi: 10.1096/fj.09-153221

Figure 4.

Figure 4.

hASCs fused with cardiomyocytes express cardiomyocyte markers 5 d after treatment with HVJ. A) Immunofluorescent staining analysis showed that hASCs fused with rat cardiomyocytes were positive for troponin I after treatment with HVJ. 1) Nuclei were stained blue with DAPI. 2) Human cells were stained green with an anti-human nuclear antibody. 3) Cardiomyocytes were stained red with an antibody against troponin I. 4) Overlay of images 1–3. Green arrow indicates the presence of human nuclei independent from rat nuclei (blue arrow) within the same cell. B) hASCs did not fuse with rat cardiomyocytes without treatment of HVJ, as evidenced by the negative expression of troponin I (red signals) in hASCs with green nuclei stained by an anti-human nuclear antibody (panel 4). 1) Nuclei were stained blue with DAPI. 2) Human cells were stained green with an anti-human nuclear antibody. 3) Cardiomyocytes were stained red with an antibody against troponin I. 4) Overlay of images 1–3. Scale bars = 50 μm.