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. 2011 Aug;25(8):2770–2781. doi: 10.1096/fj.10-169508

Figure 2.

Figure 2.

Effect of protease inhibitors on LRP-1 ectodomain shedding. Western blotting of LRP-1 α-chain in media conditioned for 24 h by fibroblastoid HT1080 cells. A) Cells were cultured without (−) or with broad-spectrum inhibitors of metalloproteinases (10 μM GM6001; 1 μM BB-94), ADAMs (1 μM KB-R7785), cysteine proteinases (20 μM E64), serine proteinases (100 μM AEBSF), or aspartyl proteinases (15 μM pepstatin). B) Cells were cultured without or with a specific inhibitor of ADAM-10 (10 μM rPA), of ADAM-17 (10 μM TAPI-1) or 1 μM human recombinant TIMP-1, TIMP-2, or N-terminal domain of TIMP-3.