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. 2011 Aug;25(8):2770–2781. doi: 10.1096/fj.10-169508

Figure 7.

Figure 7.

The two HT1080 cell variants show similar distribution of LRP-1, MT1-MMP, and ADAM-12 in Triton X-100 solubility-based fractions. Cells were extracted in cold Triton X-100 as described in Materials and Methods. A) Untreated (−) fibroblastoid and epithelioid HT1080 cells. B) Epithelioid cells were treated without (−) or with (+) MβCD to deplete cholesterol. Note that 10 μM GM6001 was added throughout the experiment to block LRP-1 ectodomain shedding. Western blotting of LRP-1 α-chain, MT1-MMP, and ADAM-12 in Triton X-100-insoluble (I) and -soluble (S) fractions of fibroblastoid and epithelioid HT1080 cells. TfR and caveolin-1 are markers of Triton X-100-soluble and -insoluble fractions, respectively. Each experimental condition was tested at least twice.