Figure 7.
p62/SQSTM1 promotes EGF-independent proliferation of PI3K-H1047R structures. (A) Phase timecourse of indicated cell types grown in 3D in the absence of EGF. Bar, 100μm. (B) Day 22 structures derived from the indicated cell types in the absence of EGF were immunostained with antibodies against Ki67 (green) and DAPI-counterstained (blue). Bar, 50μm. (C) Structures derived from the indicated cell types in the absence of EGF (day 22) were immunostained with antibodies against pERK (green) and DAPI-counterstained (blue). Bar, 50μm.