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. Author manuscript; available in PMC: 2013 Apr 11.
Published in final edited form as: Nature. 2012 Aug 1;490(7419):283–287. doi: 10.1038/nature11398

FIGURE 1. Human T cell migration in lymph nodes of BLT mice.

FIGURE 1

a. Human CD4+T cells with naïve (CD45RA+ CCR7+), central memory (CD45RA−, CCR7+) and effector memory (CD45RA−, CCR7−) phenotype are represented in lymphoid organs and peripheral blood of BLT mice. Relative frequencies in blood are similar to humans. Plots are gated on hCD4+, hCD3+ cells. b. Homing of human and murine CD4+ T cells into lymphoid tissues (pLN=peripheral, mLN=mesenteric LNs). Data pooled from three independent experiments. Values are means±s.e.m. c. Multiphoton intravital micrograph of a BLT popliteal LN 24 hours after adoptive transfer of human (green) and murine (red) T cells. Graph on the right shows migratory tracks of each population during a 30-minute recording. d, e. Mean 2D-track velocities (d) and arrest coefficients (e) of murine and human T cells in BLT LNs. Lines and numbers indicate medians. Data pooled from three recordings/two independent experiments.