Figure 3. The Morning and Evening oscillators are not necessary for daytime TPR in LD.

(A–B) The line graphs show preferred temperatures of w¹¹¹⁸ and null mutants per⁰¹ (A), and w¹¹¹⁸ and null mutants tim⁰¹ (B) during the daytime (ANOVA, P=0.77 (per⁰¹), P=0.67 (tim⁰¹)). The bar graphs show daytime TPR (ZT1–12) in LD condition for each genotype. The same LD daytime data from Figures 1B, 2F and 2H were used for w¹¹¹⁸, per⁰¹ and tim⁰, respectively. Tukey-Kramer test comparison to w¹¹¹⁸, ***P<0.001, **P<0.01 or *P<0.05.

(C–F) The line graphs show preferred temperatures of w¹¹¹⁸ and rescue fly lines in the null mutant per⁰¹ background during the daytime in LD. PER expression using tim-Gal4 (C, red) and elav-Gal4 (D, blue) are sufficient to rescue the per⁰¹ mutant phenotype for daytime TPR (ZT1–12). PER expression using cry13-Gal4 (E, green) and pdf-Gal4 (F, orange) are not sufficient to rescue the per⁰¹ mutant phenotype for daytime TPR in LD.

(G) The bar graph shows the daytime TPR (ZT1–12) in LD for each genotype shown in Figure 3A, C–F. The Morning and Evening oscillators are not sufficient. ANOVA was performed on the following groups; w¹¹¹⁸, per⁰¹;;UAS-per/+, per⁰¹;timG4/+, and per⁰¹;timG4/+;UAS-per/+ (red); as well as w¹¹¹⁸, per⁰¹;;UAS-per/+, per⁰¹;;elavG4/+ and per⁰¹;;elavG4/UAS-per (blue). Tukey-Kramer test comparison to each control fly line.

(H) The Morning and Evening oscillators are not necessary. The bar graph shows the daytime TPR (ZT1–12) in LD of flies with ablated Morning and/or Evening oscillator cells. The flies were still able to increase their preferred temperature to similar levels as the control flies during LD. The patterns of the TPR of pdf⁰¹ and pdfR^han5304 are shown in Figures S2C–D.