Table 2. Major Patterns in the Genome: Diferentially Expressed Genes in MPP+ Treated Neuroblastoma Cells after 24 Hours.
Differential whole genome expression in N2a cells treated with 500μM MPP+ vs. controls at 24 Hr.
| R [ Name, Genebank Accession #] | % Change | Direction | p-Value | ||
|---|---|---|---|---|---|
| Metabolism involving Carboxylic Acids, Transamination Reactions, Lipids, non-mitochondrial oxidative reduction and Insulin Signaling | cystathionase (cystathionine gamma-lyase) (Cth), [NM_145953] | MPP ▲ | 384% | <.001 | |
| tribbles homolog 3 (Drosophila) (Trib3), [NM_175093] | MPP ▲ | 339% | 0.004 | ||
| cytochrome b5 reductase 1 (Cyb5r1), [NM_028057] | MPP ▲ | 326% | 0.000 | ||
| 5,10-methylenetetrahydrofolate reductase (Mthfr), tv 2, [NM_010840] | MPP ▲ | 236% | 0.009 | ||
| glutamate oxaloacetate transaminase 1, soluble (Got1), [NM_010324] | MPP ▲ | 214% | 0.002 | ||
| diacylglycerol O-acyltransferase 2 (Dgat2), [NM_026384] | MPP ▲ | 213% | 0.012 | ||
| cysteine conjugate-beta lyase 1 (Ccbl1), [NM_172404] | MPP ▲ | 209% | 0.015 | ||
| pyridoxal (pyridoxine, vitamin B6) kinase (Pdxk), [NM_172134] | MPP ▲ | 206% | 0.039 | ||
| 2 | crystallin, zeta (quinone reductase)-like 1 (Cryzl1), [NM_133679] | MPP ▲ | 198% | 0.001 | |
| acetyl-Coenzyme A acyltransferase 1A (Acaa1a), [NM_130864] | MPP ▲ | 197% | 0.008 | ||
| glutamic pyruvate transaminase (alanine aminotransferase) 2 (Gpt2), [NM_173866] | MPP ▲ | 196% | 0.007 | ||
| ORM1-like 3 (S. cerevisiae) (Ormdl3), [NM_025661] | MPP ▲ | 191% | 0.041 | ||
| starch binding domain 1 (Stbd1), [NM_175096] | MPP ▲ | 190% | 0.034 | ||
| N-acetylglucosamine kinase (Nagk), tv 1, [NM_019542] | MPP ▲ | 189% | 0.017 | ||
| H2-K region expressed gene 6 (H2-Ke6), [NM_013543] | MPP ▲ | 187% | 0.023 | ||
| phosphoserine phosphatase (Psph), [NM_133900] | MPP ▲ | 180% | 0.001 | ||
| ELOVL family member 7, elongation of long chain fatty acids (yeast) (Elovl7), [NM_029001] | MPP ▲ | 175% | 0.028 | ||
| aspartate beta-hydroxylase domain containing 1 (Asphd1), [NM_001039645] | MPP ▲ | 174% | 0.026 | ||
| acyl-Coenzyme A binding domain containing 4 (Acbd4), tv 1, [NM_025988] | MPP ▲ | 172% | 0.003 | ||
| 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), [NM_008254] | MPP ▲ | 172% | 0.036 | ||
| ceroid-lipofuscinosis, neuronal 8 (Cln8), [NM_012000] | MPP ▲ | 171% | 0.013 | ||
| insulin-like growth factor binding protein 6 (Igfbp6), mRNA [NM_008344] | MPP ▲ | 261% | 0.001 | ||
| similar to 3-phosphoglycerate dehydrogenase (LOC637235), misc RNA [XR_032647] | MPP ▲ | 171% | 0.044 | ||
| ferredoxin reductase (Fdxr), nuclear gene encoding mitochondrial protein, [NM_007997] | MPP ▲ | 170% | 0.014 | ||
|
| |||||
| Angiogenesis | angiopoietin-like 6 (Angptl6), [NM_145154] | MPP ▲ | 291% | <.001 | |
| 2 | pre-B-cell leukemia transcription factor interacting protein 1 (Pbxip1), [NM_146131] | MPP ▲ | 262% | 0.028 | |
| fetuin beta (Fetub), tv 1, [NM_021564] | MPP ▲ | 255% | 0.006 | ||
| erythroid differentiation regulator 1 (Erdr1), [NM_133362] | MPP ▲ | 207% | 0.011 | ||
|
| |||||
| Transporters | solute carrier family 7 (cationic amino acid transporter, y+ system),3(Slc7a3), [NM_007515] | MPP ▲ | 285% | 0.034 | |
| solute carrier family 6 (neurotransmitter transporter, glycine), 9 (Slc6a9), [NM_008135] | MPP ▲ | 270% | 0.014 | ||
| solute carrier family 6 (neurotransmitter transporter, glycine), 9 (Slc6a9), [NM_008135] | MPP ▲ | 255% | 0.011 | ||
| purinergic receptor P2X, ligand-gated ion channel 4 (P2rx4), [NM_011026] | MPP ▲ | 235% | 0.045 | ||
| ATP-binding cassette, sub-family C (CFTR/MRP), member 5 (Abcc5), tv 1, [NM_013790] | MPP ▲ | 217% | 0.039 | ||
| recombination activating gene 1 activating protein 1 (Rag1ap1), [NM_009057] | MPP ▲ | 205% | 0.016 | ||
| solute carrier family 48 (heme transporter), member 1 (Slc48a1), [NM_026353] | MPP ▲ | 196% | 0.020 | ||
| transferrin receptor 2 (Trfr2), [NM_015799] | MPP ▲ | 191% | 0.009 | ||
| solute carrier family 7 (cationic amino acid transporter, y+ system), 5 (Slc7a5),[NM_011404] | MPP ▲ | 180% | 0.010 | ||
| solute carrier family 12, member 4 (Slc12a4), [NM_009195] | MPP ▲ | 177% | 0.013 | ||
|
| |||||
| Mitochondria | solute carrier family 25, member 33 (Slc25a33), [NM_027460] | MPP ▲ | 265% | 0.005 | |
| chloride intracellular channel 4 (mitochondrial) (Clic4), NE protein, [NM_013885] | MPP ▲ | 200% | 0.041 | ||
| lon peptidase 1, mitochondrial (Lonp1), NE protein, [NM_028782] | MPP ▲ | 196% | 0.000 | ||
| heat shock protein 1 (chaperonin 10) (Hspe1), [NM_008303] | MPP ▲ | 178% | 0.043 | ||
| NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae) NE tv 1, [NM_001170591] | MPP ▲ | 176% | 0.008 | ||
| Coenzyme A synthase (Coasy), NEM, [NM_027896] | MPP ▲ | 175% | 0.010 | ||
| ganglioside-induced differentiation-associated protein 1-like 1 (Gdap1l1) [NM_144891] | MPP ▲ | 140% | 0.002 | ||
|
| |||||
| Response to Stress or Injury | DNA-damage inducible transcript 3 (Ddit3), [NM_007837] | MPP ▲ | 414% | 0.006 | |
| protein phosphatase 1, regulatory (inhibitor) subunit 15A (Ppp1r15a), [NM_008654] | MPP ▲ | 345% | 0.014 | ||
| ChaC, cation transport regulator-like 1 (E. coli) (Chac1), [NM_026929] | MPP ▲ | 266% | 0.001 | ||
| ninjurin 1 (Ninj1), [NM_013610] | MPP ▲ | 247% | 0.033 | ||
| nuclear protein 1 (Nupr1), [NM_019738] | MPP ▲ | 225% | 0.004 | ||
|
| |||||
| G-Protein-Coupled Receptor Signaling | receptor accessory protein 6 (Reep6), [NM_139292] | MPP ▲ | 390% | 0.047 | |
| protein kinase N1 (Pkn1), [NM_177262] | MPP ▲ | 290% | 0.042 | ||
| GTP binding protein 2 (Gtpbp2), tv 1, [NM_019581] | MPP ▲ | 269% | 0.001 | ||
| arginine vasopressin-induced 1 (Avpi1), [NM_027106] | MPP ▲ | 246% | 0.024 | ||
| G protein-coupled rec associated sorting protein 2 (Gprasp2)tv 1[NM_001163015] | MPP ▲ | 228% | 0.020 | ||
| WD repeat domain 67 (Wdr67), tv 1, [NM_001081396] | MPP ▲ | 202% | 0.011 | ||
| rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2), [NM_008487] | MPP ▲ | 202% | 0.001 | ||
| folliculin interacting protein 1 (Fnip1), [NM_173753] | MPP ▲ | 198% | 0.029 | ||
| ras homolog gene family, member U (Rhou), [NM_133955] | MPP ▲ | 197% | 0.031 | ||
| syntaxin 4A (placental) (Stx4a), [NM_009294] | MPP ▲ | 194% | 0.011 | ||
| prostaglandin I receptor (IP) (Ptgir), [NM_008967] | MPP ▲ | 185% | 0.022 | ||
| pleckstrin and Sec7 domain containing (Psd), [NM_028627] | MPP ▲ | 184% | 0.013 | ||
| G protein-coupled receptor 19 (Gpr19), tv 1, [NM_001167693] | MPP ▲ | 182% | 0.024 | ||
| TBC1 domain family, member 9B (Tbc1d9b), [NM_029745] | MPP ▲ | 181% | 0.005 | ||
| folliculin (Flcn), [NM_146018] | MPP ▲ | 180% | 0.025 | ||
| ArfGAP with RhoGAP, ankyrin repeat and PH 3 (Arap3), [NM_139206] | MPP ▲ | 177% | 0.003 | ||
| Mus musculus thyroid hormone receptor interactor 10 (Trip10), [NM_134125] | MPP ▲ | 174% | 0.033 | ||
| membrane associated guanylate kinase, WW and PDZ (Magi1)tv 3[NM_001029850] | MPP ▲ | 174% | 0.041 | ||
| syntaxin 4A (placental) (Stx4a), [NM_009294] | MPP ▲ | 174% | 0.003 | ||
| ral guanine nucleotide dissociation stimulator-like 3 (Rgl3), [NM_023622] | MPP ▲ | 174% | 0.004 | ||
| T-cell lymphoma invasion and metastasis 2 (Tiam2), tv 1, [NM_011878] | MPP ▲ | 173% | 0.015 | ||
| resistance to inhibitors of cholinesterase 8 homolog (C. elegans) [NM_053194] | MPP ▲ | 172% | 0.012 | ||
| Ras-like without CAAX 1 (Rit1), tv 1, [NM_009069] | MPP ▲ | 171% | 0.035 | ||
| Rho family GTPase 1 (Rnd1), [NM_172612] | MPP ▲ | 171% | 0.010 | ||
| Rho, GDP dissociation inhibitor (GDI) beta (Arhgdib), [NM_007486] | MPP ▲ | 170% | 0.010 | ||
|
| |||||
| Signaling | phospholipase C, beta 3 (Plcb3), [NM_008874] | MPP ▲ | 172% | 0.010 | |
| lymphocyte protein tyrosine kinase (Lck), tv 2, [NM_010693] | MPP ▲ | 225% | 0.002 | ||
| protein kinase C, delta (Prkcd), [NM_011103] | MPP ▲ | 217% | 0.029 | ||
| phospholipase C, delta 3 (Plcd3), [NM_152813] | MPP ▲ | 200% | 0.015 | ||
NE = Nuclear gene encoding mitochondrial protein
The data represents genes found to be up-regulated by MPP+ expressed by functional category, gene identification, fold change and significance at p < 0.05. R=gene probe replicates in hybridization array.