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. Author manuscript; available in PMC: 2013 Nov 2.
Published in final edited form as: Oncogene. 2012 Jul 9;32(18):2249–226121. doi: 10.1038/onc.2012.237

Figure 1.

Figure 1

Characterization of reprogrammed-sarcomas as compared to parental sarcomas: (A–E) (i) parental cell in culture (scale bar=50um), (ii-top) embryoid body formation (scale bar=500um); (ii-bottom) reprogrammed cells in culture (scale bar=50um), (iii), expression via RT-PCR of reprogramming transcription factors in parental (PAR) and reprogrammed (REP) sarcomas; (iv) ESC markers via immunofluorescence in reprogrammed cells, and lineage directed differentiation (via Activin A (50ng/ml) or Noggin (100ng/ml) directed differentiation into endoderm and ectoderm respectively; (scale bar=500um); (v) morphologically and via expression of lineage specific markers (e.g., Sox 17 - endoderm, Pax6 ectoderm) (scale bar=50um). All cells were grown on matrigel. (F) Quantification of Ki67 and vimentin immunohistochemistry of xenografts from parental and reprogrammed cells as seen in Supplemental Figure 5.