Figure 2.

(A) Agonist-induced calcium mobilization in a CHO cell line stably expressing human MCH-2R as detected by a fluorometric imaging plate reader (FLIPR) assay. MCH (●), Phe¹³Tyr¹⁹-MCH (○), salmon MCH (▴). (B) Membrane binding assay using a scintillation proximity assay measuring specific binding of [¹²⁵I]Phe¹³Tyr¹⁹-MCH to MCH-2R containing membranes. (C and D) PTX sensitivity of intracellular calcium mobilization mediated by MCH-1R (C) or MCH-2R (D) in HEK293-AEQ17 cell lines. Stable transfected HEK293-AEQ17 cell lines were treated with 0 ng/ml (circles), 150 ng/ml (triangles), 500 ng/ml (diamonds), or 5,000 ng/ml (rectangles) PTX before the aequorin assay.