Table 1.

Comparison of half-maximal concentrations for binding at and functional activation of human MCH-1R and MCH-2R by MCH, Phe¹³Tyr¹⁹-MCH, and salmon MCH

Ligand	Binding IC50, nM ± SEM (n)		Function EC50, nM ± SEM (n)
	MCH-1R	MCH-2R	MCH-1R	MCH-2R
MCH	0.3  ± 0.1 (10)	1.5  ± 0.9 (8)	3.9  ± 1.2 (3)	0.1  ± 0.1 (3)
Phe13Tyr19-MCH	0.3  ± 0.1 (3)	0.8  ± 0.1 (3)	8.5  ± 0.9 (3)	0.2  ± 0.1 (3)
Salmon MCH	0.2  ± 0.1 (5)	436.7  ± 143.8 (3)	35.0  ± 14.8 (3)	0.8  ± 0.1 (3)

Membrane binding was assessed by competition of [¹²⁵I]Phe¹³Tyr¹⁹-MCH in a scintillation proximity assay. Functional activation was measured by mobilization of intracellular calcium detected in a fluorometric imaging plate reader assay. Values shown are nanomolar concentrations and averaged from at least three independent experiments.