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Effect of controlled freezing rate on bulk glycerol frozen samples. Recovery of RBCs preserved by various glycerol freezing and thawing methods over a period of two weeks (mean ± SD). Controlled freezing rate (n=2; Δ) compared to regular bulk glycerol freezing (n=2; □) with standard thawing protocol, and controlled freezing rate (n=4; ▲) compared to regular bulk glycerol freezing (n=4; ■) with the modified thawing protocol.
