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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Cell Physiol. 2013 Feb;228(2):349–361. doi: 10.1002/jcp.24138

Figure 1. Flow cytometric analysis of osteoclast culture purity.

Figure 1

BM-OCs were stimulated with RANK-L for 72hrs on UpCell plates. Cells were probed for RANK (PerCP-Cy5.5) and calcitonin receptor [CTR] (AlexaFluor647) and acquired using a FACSCalibur flow cytometer. FCS Express software was used to determine forward and side scatter population of analyzed BM-OCs (circled in upper panel) and percentage of pure RANK+CTR+ BM-OCs (lower panel). Black arrow indicates novel population of RANK+ cells expressing low levels of CTR [RANK+CTRlo] NOD BM-OCs. Data shown as representative scatter plots of each mouse strain.