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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Cell Physiol. 2013 Feb;228(2):349–361. doi: 10.1002/jcp.24138

Figure 2. NOD-derived osteoclasts display altered differentiation.

Figure 2

(A) 6d post-differentiation, BM-OCs were imaged with light microscopy at 40x magnification. Representative images of (I) mononuclear cells, (II) multinucleated OCs, and (III) giant OCs. (B-D) Number of TRAP+ BM-OCs per coverslip were enumerated using 40x magnification. (E-G) Data shown as percentage of total nuclei TRAP+. C57Bl/6 (black bars, n=18), Balb/c (dark grey bars, n=16), NOR (light grey bars, n=16), NOD (white bars, n=16). (H) 6d and 9d post-differentiation, multinucleated and giant BM-OCs NOD mice were quantified. (I) 6d and 9d post-differentiation cell-viability of C57Bl/6 and NOD BM-OC cultures was assessed by MTT assay. *p value < 0.05. One-way ANOVA with Dunn’s multiple correction.