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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Cell Physiol. 2013 Feb;228(2):349–361. doi: 10.1002/jcp.24138

Figure 6. NOD-derived osteoclasts secrete increased soluble osteoclastogenic mediators in response to LPS.

Figure 6

BM-OCs were stimulated with RANK-L in the presence (+) or absence (-) of E. coli LPS for 72hrs on bovine bone slices. (A, G) GM-CSF (B, H) IL-1β (C, I) TNF-α, (D. J) RANTES, (E, K) IP-10 and (F, L) IL-10 levels were evaluated in the permeabilized supernatants using Milliplex technology. (A-F) Data shown as pg/mL normalized to TRAP+ mononuclear cells. (G-L) Data shown as pg/mL normalized to TRAP+ multinucleated cells. C57Bl/6 (black bars, n=13), Balb/c (dark grey bars, n=15), NOR (light grey bars, n=14), NOD (white bars, n=17). Open bars = absence of LPS, hatched bars = presence of LPS. *p value ≤ 0.05. One-way ANOVA with Dunn’s multiple correction.