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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Dev Biol. 2012 Aug 11;371(1):47–56. doi: 10.1016/j.ydbio.2012.08.003

Figure 5. Whole-mount in situ hybridization (ISH) analysis in E10.5 Edn1fl/fl conditional knockout embryos.

Figure 5

Ventral view of embryos after ISH for Dlx2 (A, F, K, P, U), Dlx5 (C, H, M, R, W), Hand2 (D, I, N, S, X) and Goosecoid (E, J, O, T, Y). The heart has been removed to aid in visualization. B, G, L, Q, V show a lateral view of embryos after ISH for Dlx3. A–E. Expression in E10.5 control embryos. F–J. In Edn1fl/fl;Foxg1-Cre embryos, Dlx2 expression was disrupted in the distal ectoderm of the mandibular first pharyngeal arch (arrows in F). Dlx3 expression was almost completely lost in the first pharyngeal arch and reduced in the second pharyngeal arch (arrow in G). Dlx5 expression was disrupted, but only in a band across the central mandibular arch (asterisks in H). Hand2 (I) and Goosecoid (J) expression appeared unaffected. K–O. Dlx5 expression in Edn1fl/fl;Crect embryos was also disrupted (asterisks in M).Expression of other genes examined was unaffected. P–Y. The expression of all five genes was unaffected in Edn1fl/fl;Myf5-Cre (P–T) and Edn1fl/fl;Foxa2mcm (U–Y) embryos. 1, mandibular pharyngeal arch; 2, second pharyngeal arch.