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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Dev Biol. 2012 Aug 11;371(1):47–56. doi: 10.1016/j.ydbio.2012.08.003

Figure 8. Nkx3.2 expression is disrupted in the intermediate mandibular arch domain of Edn1fl/fl;Crect embryos.

Figure 8

Ventral view of E10.5 Edn1fl/fl;Crect embryos after single (A–D) or double (E–F) ISH for Dlx5 and/or Nkx3.2. A–B. Dlx5 expression is disrupted in a strip of the mandibular arch mesenchyme in Edn1fl/fl;Crect embryos (B). C–D. Mesenchymal expression of Nkx3.2 is disrupted in the mandibular of Edn1fl/fl;Crect embryos, while epithelial expression (arrow) is unaffected (D). E–F. Double ISH for Dlx5 (magenta) and Nkx3.2 (blue) expression. In control embryos, mesenchymal expression of both genes overlaps (E). In Edn1fl/fl;Crect embryos, mesenchymal Nkx3.2 expression is largely absent from the domain in which Dlx5 expression has been lost (F). 1, mandibular pharyngeal arch; 2, second pharyngeal arch.