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. 2012 Oct 3;2012:341479. doi: 10.1155/2012/341479

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Copyright © 2012 Chih-Ping Hsu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunoblots of apoptosis-controlling proteins in LCSP-treated colorectal carcinoma cells. The cell lysates from LCSP-treated SW480 (a) and Colo320DM (b) were separated by SDS-PAGE, transferred to PVDF membranes, and immunoblotted to show the levels of Bax, Bcl-2, procaspase 3 and cleavage-PARP. The protein levels of Bax and Bcl-2 were quantified using ImageJ software according to the density of each band on the immunoblotting image, normalized to the reference band (β-actin), and presented as the fold of the untreated control. (c) The data reported are the averages of three independent experiments and are expressed as means ± SD. *Represents a significant difference (P < 0.05).