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Kinetic model of the Tetrahymena ribozyme reaction. The free enzyme (E) can bind the oligonucleotide substrate (S) to form the open complex, in the presence [(E·S·G)O] or absence [(E·S)O] of guanosine. The closed complex [(E·S·G)C or (E·S)C] forms when the P1 helix carrying the substrate “docks” into the active site of the ribozyme via the formation of tertiary interactions. [Taken from (111).]
