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Human CASMCs express vitamin D receptor (VDR), but not calcium sensing receptor (CaSR), mRNA by RNase protection assays. A: The 282 nt VDR probe protected a 184 nucleotide (nt) fragment. B: The 540 nt CaR probe protected a 448 nt fragment. The 138 nt cyclophilin (cyclo) probe protected a 103 nt fragment. Lane 1: Probe; lanes 2 and 3: CASMC lot number 3F0246; lane 4: CASMC lot number 00077; lane 5: yeast (negative control); lane 6: kidney (positive control).
