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Kinetic parameters for TbPdxK with B6 vitamers and ginkgotoxin
| Substrate | Kmapp (µM) | kcat (s−1) | kcat/Kmapp (M−1 s−1) |
|---|---|---|---|
| Pyridoxal | 14.8 ± 0.7 | 27.3 ± 1.3 | 1.84 × 107 |
| Pyridoxine | 48.0 ± 12.7 | 122 ± 7 | 2.54 × 107 |
| Pyridoxamine | 59.4 ± 14.8 | 127 ± 15 | 2,14 × 107 |
| Ginkgotoxin | 46.6 ± 10.2 | 106 ± 2 | 2.28 × 107 |
Details of the coupled assay system are described in Experimental procedures.
