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. 1982 Oct;79(20):6285–6288. doi: 10.1073/pnas.79.20.6285

Properties of a cell-free system for synthesis of citrus exocortis viroid

Ricardo Flores 1,*, J S Semancik 1
PMCID: PMC347105  PMID: 16593239

Abstract

Partially purified nuclei from citrus exocortis viroid (CEV)-infected Gynura aurantiaca are able to synthesize linear and circular viroid molecules. Pretreatment of the nuclei with actinomycin D or deoxyribonuclease did not affect viroid synthesis, whereas the synthesis of other cellular RNAs was severely reduced. These observations support the essential role of CEV complementary RNA sequences in viroid replication. However, when α-amanitin was included in this in vitro synthesis system, CEV replication was markedly reduced by concentrations of 10 nM or greater. Taken together, these data support the proposition that viroid synthesis is catalyzed by a DNA-dependent RNA polymerase acting on a RNA template.

Keywords: nuclei-rich fraction, actinomycin D, DNase, α-amanitin, denaturing gel

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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