Table 2.
|
|
Distance of transection (μm) |
|
|
|
||
---|---|---|---|---|---|---|---|
|
|
25 |
150 |
300 |
Sham |
Control |
|
No. of neurons | 131 | 106 | 96 | 94 | 132 | p<0.05 | |
Degeneration | Length (μm) | 22.9±0.5 | 89.1±28.6 | 152.6±9.0 | 150 and 300 versus 25; 150 versus 300 | ||
Rate (μm/h) | 14.5±0.9 | 42.6±5.4 | 63.3±12.2 | 150 and 300 versus 25; 150 versus 300 | |||
Regeneration | 1 (0.8%) | 13 (12.3%) | 14 (14.6%) | 150 and 300 versus 25 | |||
Starting time (min) | 120 | 218.5±31.1 | 141.7±14.8 | 150 versus 300 (25 not included in analysis because of small sample size) | |||
Length (μm) | 27 | 49.1±7.4 | 69.1±9.0 | ||||
Rate (μm/h) | 4.5 | 15.2±4.5 | 14.6±2.1 | ||||
Branching | 1 (100%) | 6 (46.2%) | 7 (50%) | ||||
Death | Rate | 60 (45.8%) | 19 (17.9%) | 19 (19.8%) | 0 | 10 (7.6%) | 25, 150, and 300 versus sham and control; 150 and 300 versus 25 |
Time (min) | 229.1±25.2 | 262.3±80.9 | 408.3±79.6 | 339±47 | 25 versus 300 | ||
Apoptotic | 25 (19.1%) | 5 (4.7%) | 6 (6.3%) | 5 (3.8%) | |||
Necrotic | 35 (26.7%) | 14 (13.2%) | 13 (13.5%) | 5 (3.8%) |