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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: Nat Struct Mol Biol. 2010 Dec 5;18(1):75–79. doi: 10.1038/nsmb.1942

Figure 4.

Figure 4

CtIP binds to switch region DNA. (a) ChIP was carried out in CIT-stimulated CH12 cells with AID, CtIP or control non-specific IgG antibodies. DNA from the ChIP samples was diluted 3-fold, amplified by PCR and the presence of Sµ, µpromoter (Iµ promoter) or p53 was determined by analyzing the PCR products on agarose gels. (b) The amount of CtIP bound to Sµ in the indicated cells was measured by real-time quantitative PCR. Data are the mean of three independent experiments with error bars representing standard deviation from the mean. (c) CtIP binding to Sµ in unstimulated or CIT-stimulated CH12 cells was assayed by ChIP and quantified by real-time PCR. The values represent the mean of three independent experiments with error bars representing standard deviation from the mean. (d) Splenic B cells from wild type or AID−/− mice were stimulated with α-CD40 and IL-4 for 48hrs and ChIP was performed with CtIP, AID, H3 and control non-specific IgG antibodies. Immunoprecipitated DNA was diluted 4-fold and amplified by PCR for the presence of Sµ, Sγ1, Iµ promoter or p53.