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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: Oncogene. 2008 Jan 28;27(25):3615–3623. doi: 10.1038/sj.onc.1211018

Figure 6.

Figure 6

Decitabine (DAC)-induced G2/M arrest is p53 dependent and p21WAF1/CIP1 upregulation is ATM dependent. (a)Synchronize d HL-60 cells were treated with DAC (0.5, 1 μM)for 48 h and cell cycle analysis was performed as described under Materials and methods. Results represent the mean±s.d. for three independent experiments. (b)HL-60 and ML-1 cells were treated with DAC (500, 1000 nM)for 72 h. Apoptosis was measured as described under Materials and methods. Results represent the mean±s.d. for three independent experiments. (c)ML-1 cells were treated with DAC (1, 2 μM), caffeine (1mM), MS-275 (1 μM)or cotreated with DAC + caffeine or MS-275 + caffeine for 48 h. The expression of p21WAF1/CIP1 was measured by western blotting. Actin was used as a loading control. Numerical values above each blot represent the signal intensity measured by densitometry. (d)ML-1 cells were treated with DAC (1 μM), caffeine (1mM), MS-275 (1 μM), KU-5593 (20 μM)alone or in combination for 48 h. Caffeine was used as a cotreatment, while KU-5593 was used as a pretreatment for 1 h.