Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Oct 15;199(2):317–329. doi: 10.1083/jcb.201201157

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Wynne et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 7. — Integrated structure of the RA-PH domains is required for PM association of RIAM. (A) Schematic representation of RIAM RA-PH domain tandem probes used in B–E. An X through a domain indicates that it has been inactivated by mutation (K213A for RA and K327A/K331A/R333A for PH). (B and C) Localization of GFP-2xRAPH expressed alone in Jurkat T cells (B) or with mCherry-Rap1V12 (C). (D) Localization of the indicated GFP-tagged tandem RA-PH probes in Jurkat T cells coexpressing mCherry-Rap1V12 at near endogenous levels or active HrasV12 expressed 10–100-fold above endogenous (grayscale images to right). (E) Chart summarizing PM recruitment. Bars: 5 µm.