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. 2012 Oct 15;199(2):347–363. doi: 10.1083/jcb.201203134

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© 2012 Amelio et al.

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Figure 2. — In vivo inhibition of miR-24 expression increases cell proliferation. (A) In situ hybridization analysis reveals reduction of endogenous miR-24 by antagomir-24 subcutaneous injections in newborn mice dorsal skin. Dotted lines delineate the basal layer of the epidermis. Bars, 100 µm. (B) Real-time qPCR performed on RNA extracts from skin of treated mice quantifies endogenous miR-24 silencing. Histogram shows means ± SD of miR-24 relative levels in treated dorsal skin (n = 4 mice for each group). (C and D) In vivo miR-24 silencing results in the induction of proliferation. Skin from antagomir-24–treated mice showed an increase in BrdU-positive cells in the basal layer (n = 4 mice for each group). Dotted boxes indicate the insets magnified on the bottom. Bars, 30 µm. Epi, epidermis; Der, dermis; Scr, scramble. *, P < 0.001.