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. 2012 Oct 15;199(2):347–363. doi: 10.1083/jcb.201203134

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© 2012 Amelio et al.

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Figure 4. — miR-24 targets PAK4, Tks5, and ArhGAP19 3′UTRs. (A) PAK4, Tks5, and ArhGAP19 are down-regulated during calcium-induced keratinocyte differentiation (diff.). (B) Histograms show densitometry analysis of the Western blot in A. (C) Insertion of the 3′UTR from miR-24 putative targets decreases luciferase reporter activity (*, P < 0.01), whereas deletion of miR-24 seed sequences from target 3′UTRs abrogates the repression. (D) miR-24 in vitro and in vivo regulates PAK4, Tks5, and ArhGAP19 protein levels. (left) 48 h after transfection, HEKn were collected to perform Western blot analysis. Skin samples of antagomir-24–treated mice (from the experiment shown in Fig. 2) were used to perform Western blot analysis. Four antagomir-24–-injected mice (lanes 1–4) and four scramble-injected mice (lanes 5–8) were analyzed. Error bars show means ± SD. MM, molecular mass; Scr, scramble; Luc, luciferase; Mt, mutant.