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. 2012 Oct 15;199(2):285–301. doi: 10.1083/jcb.201203091

Figure 7.

Figure 7.

CLASP2 C-terminal phosphorylation by Plk1 is required for normal chromosome alignment and mitotic duration. (A) CLASP2 Western blot of control and endogenous CLASP2-depleted HeLa cells expressing GFP-CLASP2 S_A Plk1 mutant. (B) Quantification of HeLa mCherry-H2B Histone cells with delay in chromosome alignment in control (n = 27), CLASP2-depleted cells (n = 29), and cells depleted of CLASP2 expressing GFP-CLASP2 S_A Plk1 mutant (n = 16). The results represent a pool from at least three independent experiments. (C) Quantification of mean NEB-anaphase duration in HeLa mCherry-H2B Histone control (n = 27), CLASP2-depleted (n = 29), and cells depleted of CLASP2 expressing GFP-CLASP2 S_A Plk1 mutant (n = 16). Error bars represent standard deviations from at least three independent experiments; *, P < 0.05, Student t test. (D) Selected frames of representative videos of HeLa mCherry-H2B Histone (red) cells in control, CLASP2-depleted with, and CLASP2-depleted without GFP-CLASP2 S_A Plk1 mutant expression (green). Arrowheads indicate chromosomes with delayed alignment. Time is shown in hours:minutes. Bar, 10 µm.