Systemic TM therapy remodels mammary gland ductal morphogenesis in Her2/neu transgenic and FVB mice. A, mammary gland ductal network. Mammary glands from control and TM-treated Her2/neu and FVB mice were isolated and processed for whole mount analysis using carmine-red staining. The complexity of mammary ductal branching was quantified using carmine-red stained mammary glands to count the number of secondary branches in three representative fields (100X). Mean number of secondary branches per field was calculated and presented as mean ± SEM. *, p-value <0.05. B, mammary gland histology. Mammary glands from control and TM-treated Her2/neu and FVB mice were isolated, fixed, and processed for hematoxylin and eosin staining. Mean number of mammary epithelial cells per field (100X) was calculated and presented as mean ± SEM.