Table 2.
Effects of α-chloralose anesthesia and l-NAME on CBF and glucose utilization in some representative cerebral structures outside whisker-to-barrel cortex sensory pathway
| Structure and treatment | CBF, ml/100 g/min
|
Cerebral glucose utilization, μmol/100 g/min
|
||||||
|---|---|---|---|---|---|---|---|---|
| n | Left side | Right side | % L-R diff. | n | Left side | Right side | % L-R diff. | |
| Cerebellar white matter | ||||||||
| Conscious + saline | 6 | 50 ± 2 | 50 ± 1 | 2 ± 3 | 6 | 33 ± 2 | 34 ± 2 | 3 ± 2 |
| Conscious + l-NAME | 6 | 34 ± 2** | 35 ± 1 | 2 ± 4 | 6 | 31 ± 1 | 31 ± 1 | 0.3 ± 2 |
| Anesthetized + saline | 7 | 24 ± 2** | 24 ± 3 | −1 ± 4 | 5 | 18 ± 1** | 18 ± 1 | −2 ± 2 |
| Anesthetized + l-NAME | 6 | 18 ± 1 | 18 ± 1 | 4 ± 3 | 5 | 19 ± 1 | 18 ± 1 | −6 ± 2 |
| Caudate-putamen | ||||||||
| Conscious + saline | 6 | 164 ± 13 | 165 ± 11 | 0.5 ± 2 | 6 | 120 ± 3 | 121 ± 4 | 1 ± 1 |
| Conscious + l-NAME | 6 | 139 ± 8 | 138 ± 9 | −1 ± 2 | 6 | 129 ± 4 | 129 ± 4 | −0.2 ± 1 |
| Anesthetized + saline | 7 | 68 ± 5** | 66 ± 6 | −4 ± 3 | 5 | 44 ± 3** | 43 ± 3 | −1 ± 1 |
| Anesthetized + l-NAME | 6 | 47 ± 1† | 44 ± 2 | −6 ± 2 | 5 | 49 ± 1 | 47 ± 1 | −3 ± 2 |
| Motor cortex | ||||||||
| Conscious + saline | 6 | 165 ± 10 | 197 ± 17 | 19 ± 5 | 6 | 103 ± 2 | 108 ± 3 | 5 ± 2 |
| Conscious + l-NAME | 6 | 139 ± 11 | 152 ± 14 | 9 ± 3 | 6 | 110 ± 3 | 115 ± 3 | 4 ± 1 |
| Anesthetized + saline | 7 | 58 ± 4** | 57 ± 4 | −1 ± 1 | 5 | 37 ± 3** | 37 ± 3 | 2 ± 2 |
| Anesthetized + l-NAME | 6 | 42 ± 1† | 42 ± 1 | 1 ± 3 | 5 | 42 ± 2 | 41 ± 2 | −2 ± 2 |
| Nucleus accumbens core | ||||||||
| Conscious + saline | 6 | 172 ± 11 | 171 ± 14 | −1 ± 3 | 6 | 72 ± 2 | 73 ± 3 | 1 ± 2 |
| Conscious + l-NAME | 6 | 117 ± 13* | 112 ± 11 | −4 ± 3 | 6 | 76 ± 4 | 74 ± 3 | −2 ± 1 |
| Anesthetized + saline | 5 | 59 ± 8** | 59 ± 8 | −1 ± 2 | 5 | 32 ± 3** | 32 ± 3 | −1 ± 1 |
| Anesthetized + l-NAME | 6 | 34 ± 2 | 36 ± 1 | 5 ± 4 | 5 | 33 ± 1 | 33 ± 1 | −1 ± 2 |
Values are means ± SEM of number of animals indicated. Percent L-R diff. (left-right difference) is the mean of the individual side-to-side percent differences obtained in each rat and not the percent difference between the means. Conscious saline-treated controls vs. anesthetized saline-treated or conscious l-NAME-treated groups (only left side statistically analyzed),
*
, P < 0.05;
**
, P < 0.01;
**
, P < 0.001 (Bonferroni corrected). Anesthetized saline-treated vs. anesthetized l-NAME-treated groups (only left side statistically analyzed),
†
, P < 0.05 (Bonferroni corrected).