Figure 4.
(Color online). Immune effects of washed VC. Three mice were instilled endotracheally with either saline or 106cfu per mouse of VC, and were sacrificed at 2-h post-exposure and lung sections were stained with rat anti-mouse-LY-6G and anti-rat-TRITC antibodies. Examples of confocal micrographs shown in (a) (saline) and (b) (Bc VC) were used to enumerate three random fields from each lung section of three mice (nine fields total per group) of LY-6G-positive cells (red-orange), and are summarized with box-whisker plots in (c). Box limits represent the 25th and 75th percentiles and whiskers represent the 5th and 95th percentiles. Means (horizontal line within boxes) are for 10 individual counts (solid circles). Pulmonary pyrogenic cytokine levels (d) were derived from homogenized lung tissue using a multiplex bead array system against antigenic standards supplied by the manufacturer. Error bars represent the standard deviation. Asterisks indicates significantly different values from pooled saline exposures, as determined using ANOVA and Dunnett's Multiple Comparison Test (∗p < 0.1; ∗∗p < 0.05).