Table 1.
Cytokine levels (pg mL−1) 2h after Bc14579 vegetative cell exposure.
| Dose (cfu per 25 μL) | IL-1β | IL-6 | TNF-α |
|---|---|---|---|
| Lung cytokines | |||
| 0 (vehicle) | 614.6±545.5 | 47.6±26.8 | 15.0±2.4 |
| 520.5±74.6 | 60.8±24.5 | 20.4±1.8 | |
| 103 | 322.07±91.0 | 42.4±26.5 | 12.2±3.0 |
| 104 | 592.6±387.6 | 43.0±24.1 | 9.8±3.8 |
| 105 | 6515.4±4571.1 | 6336.3±5226.7 | 181.5±169.8 |
| 106 | 6873.0±6321.9 | 9520.2±9478.3 | 271.6±379.6 |
| Plasma cytokines | |||
| 0 (vehicle) | Not detected | 4.6±1.4 | 0.0±0.0 |
| 102 | Not detected | 5.5±4.5 | 0.7±0.0 |
| 103 | Not detected | 3.5±0.9 | 0.7±0.0 |
| 104 | Not detected | 5.9±0.9 | 0.7±0.4 |
| 105 | Not detected | 133.6±149.6 | 3.3±3.6 |
| 106 | Not detected | 122.2±137.6 | 4.3±0.0 |
Notes: Mice (three per group) instilled endotracheally with either saline or various doses of VCs were sacrificed at 2-h post-exposure. Pyrogenic cytokines were measured in homogenized portions of lung tissue and plasma using a multiplex bead array system. The high standard deviation in some exposures resulted from usually one non-responsive animal per group.