Figure 1. Myc-EGFP expression during lymphocyte activation (see also Figure S1).
(A) Activation and phosphorylation of the c-Myc-EGFP knock-in. Immunoblot analysis of extract from (i) B splenocytes at 0, 1.5, 3.0, 4.5 and 6.0 hours post-LPS activation and (ii) from ConA activated T splenocytes at 0, 1.5, 4, 5, 8, 11, 14 and 18 hours.
(B) Flow cytometric analysis of (i) LPS activated c-Myc GFP/GFP splenic B cells at 0, 2, 4, 5, 6 and 7 hours and (ii) of ConA activated c-Myc GFP/GFP splenic T cells at 0, 1.5, 3, 4, 5, 8, 11, 14, 17 and 20 hours. The scale and axes are indicated in the left bottom corner.