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. Author manuscript; available in PMC: 2013 Sep 28.
Published in final edited form as: Cell. 2012 Sep 28;151(1):68–79. doi: 10.1016/j.cell.2012.08.033

Figure 1. Myc-EGFP expression during lymphocyte activation (see also Figure S1).

Figure 1

(A) Activation and phosphorylation of the c-Myc-EGFP knock-in. Immunoblot analysis of extract from (i) B splenocytes at 0, 1.5, 3.0, 4.5 and 6.0 hours post-LPS activation and (ii) from ConA activated T splenocytes at 0, 1.5, 4, 5, 8, 11, 14 and 18 hours.

(B) Flow cytometric analysis of (i) LPS activated c-Myc GFP/GFP splenic B cells at 0, 2, 4, 5, 6 and 7 hours and (ii) of ConA activated c-Myc GFP/GFP splenic T cells at 0, 1.5, 3, 4, 5, 8, 11, 14, 17 and 20 hours. The scale and axes are indicated in the left bottom corner.